1. The blood is drawn directly from the vein into a special EOT®II syringe. Glass beads with uneven surfaces within the EOT®II syringe make the blood cells adhere to them and encourage the synthesis of inflammation block proteins and growth factors.
2. EOT®II syringes are placed in an incubator at 37C, for 6-9 hours, which represents optimal conditions for obtaining the substances produced during the physiological healing of injury:
– inflammation blockers cytokines (e.g. IL-1Ra, IL-10, signal proteins which may restrict the intensity and duration of an inflammation of the tissue and kick-start regeneration) and lipid mediators;
– growth factors and exosoma (stimulate regeneration in the affected tissue).
The totality of these substances comprises Blood Cell Secretome (BCS).
3. Blood cells are separated from serum by centrifugation and the serum enriched with a high concentration of inflammation blocking proteins, lipid mediators, growth factors and exosoma is kept for further use.
4. Finally, we get 5-6 serum injections, which, depending on the indication, are injected 1-2 times a week into the affected joint of another region of the body.